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sdf 1α  (R&D Systems)


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    Structured Review

    R&D Systems sdf 1α
    Sdf 1α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+cxcl12+sdf+1%CE%B1/Recombinant+Human%2FRhesus+Macaque%2FFeline+CXCL12%2FSDF-1a%2C+CF/pm29456793__ml7b00406_si_001-29-0-1
    Average 94 stars, based on 88 article reviews
    sdf 1α - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Microtubules Regulate Migratory Polarity through Rho/ROCK Signaling in T Cells
    Article Snippet: .. Recombinant human CXCL12/SDF-1α and ICAM-1/Fc chimera were purchased from R&D Systems (Abingdon, UK). .. Nocodazole, taxol (paclitaxel), FITC-conjugated anti-α-tubulin antibody (DM1A), anti-acetylated tubulin antibody (6-11B-1) and TRITC-conjugated-phalloidin were purchased from Sigma-Aldrich (St. Louis, MO, USA), anti-phospho-Akt (Thr308 and Ser473), anti-Akt, anti-phospho-ERM and anti-ERM, anti phospho-cofilin (Ser3), anti-cofilin and anti-stathmin/Op18 antibodies from Cell Signaling (Danvers, MA, USA), anti-ICAM3 antibody from Abcam (Cambridge, UK), anti-Glu-tubulin antibody from Chemicon (Chandlers Ford, UK), blebbistatin, Y-27632 and H-1152 from Calbiochem (Nottingham, UK), Alexa546-conjugated phalloidin, Alexa488, Alexa546-conjugated goat anti-rabbit IgG (H+L) and Alexa488-conjugated goat anti-mouse IgG (H+L) from Invitrogen (Paisley, UK), goat anti-rabbit IgG (H+L)-HRP and goat anti-mouse IgG (H+L)-HRP from GE Healthcare UK Limited (Bucks, UK), anti-phospho-stathmin/Op18 (Ser16) and anti-RhoA antibody (26C4) from Santa Cruz (Autogen Bioclear UK Ltd, Wiltshire, UK).

    Article Title: Simultaneous Inhibition of CXCR4 and VLA-4 Exhibits Combinatorial Effect in Overcoming Stroma-Mediated Chemotherapy Resistance in Mantle Cell Lymphoma Cells
    Article Snippet: Cultures were maintained by removing medium, rinsing with 0.25% Trypsin-EDTA solution (Gibco by Life Technologies) followed by the addition of a fresh culture medium every 2 to 3 days. .. Recombinant human CXCL12 (SDF-1α) was purchased (R&D Systems, Minneapolis, MN, USA). .. The following monoclonal Abs specific for human surface antigens were used: Purified NA/LE mouse anti-human CD184, FITC anti-human CD49d and FITC mouse anti-human CD45 (BD Pharmingen, San Jose, CA, USA).

    Article Title: Targeting CXCR4 abrogates resistance to trastuzumab by blocking cell cycle progression and synergizes with docetaxel in breast cancer treatment
    Article Snippet: .. Materials And Methods Drugs and reagents AMD3100 and cisplatin were purchased from Selleckchem (Houston TX); trastuzumab, docetaxel and carboplatin from MD Anderson Cancer Center Pharmacy; and recombinant human CXCL12/SDF-1α from R&D Systems (Minneapolis, MN). .. Cell culture and generation of stable cell lines BT474 and SKBR3 cell lines were obtained from the American Type Culture Collection (Manassas, VA).

    Article Title: Injectable myocardium-derived hydrogels with SDF-1α releasing for cardiac repair.
    Article Snippet: Myocardial infarction (MI) is a predominant cause of morbidity and mortality globally.. Therapeutic chemokines, such as stromal cell-derived factor 1α (SDF-1α), present a promising opportunity to treat the profibrotic remodeling post-MI if they can be delivered effectively to the injured tissue.. However, direct injection of SDF-1α or physical entrapment in a hydrogel has shown limited efficacy.

    Article Title: Targeting CXCR4 abrogates resistance to trastuzumab by blocking cell cycle progression and synergizes with docetaxel in breast cancer treatment
    Article Snippet: .. AMD3100 and cisplatin were purchased from Selleckchem (Houston TX); trastuzumab, docetaxel, and carboplatin from MD Anderson Cancer Center Pharmacy; and recombinant human CXCL12/SDF-1α from R&D Systems (Minneapolis, MN). .. BT474 and SKBR3 cell lines were obtained from the American Type Culture Collection (Manassas, VA).

    Article Title: Targeting CXCR4 abrogates resistance to trastuzumab by blocking cell cycle progression and synergizes with docetaxel in breast cancer treatment.
    Article Snippet: .. AMD3100 and cisplatin were purchased from Selleckchem (Houston TX); trastuzumab, docetaxel, and carboplatin from MD Anderson Cancer Center Pharmacy; and recombinant human CXCL12/SDF-1α from R&D Systems (Minneapolis, MN). .. Cell culture and generation of stable cell lines BT474 and SKBR3 cell lines were obtained from the American Type Culture Collection (Manassas, VA).

    Article Title: Injectable myocardium-derived hydrogels with SDF-1α releasing for cardiac repair
    Article Snippet: Cell counting kit 8 (CCK-8) was received from Dojindo. .. Recombinant human CXCL12/SDF-1α and human CXCL12/SDF-1α ELISA kit were obtained from R&D systems. .. Poly(lactic- co -glycolic acid) (55–65 kDa, 50:50) was purchased from Akina Polyscitech.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Injectable myocardium-derived hydrogels with SDF-1α releasing for cardiac repair.
    Article Snippet: Myocardial infarction (MI) is a predominant cause of morbidity and mortality globally.. Therapeutic chemokines, such as stromal cell-derived factor 1α (SDF-1α), present a promising opportunity to treat the profibrotic remodeling post-MI if they can be delivered effectively to the injured tissue.. However, direct injection of SDF-1α or physical entrapment in a hydrogel has shown limited efficacy.

    Article Title: Injectable myocardium-derived hydrogels with SDF-1α releasing for cardiac repair
    Article Snippet: Cell counting kit 8 (CCK-8) was received from Dojindo. .. Recombinant human CXCL12/SDF-1α and human CXCL12/SDF-1α ELISA kit were obtained from R&D systems. .. Poly(lactic- co -glycolic acid) (55–65 kDa, 50:50) was purchased from Akina Polyscitech.



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    Image Search Results


    GBM: Glioblastoma multiforme; Alg 1%: Alginate hydrogel 1%; Alg/Chit-NPs CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); CXCL12: C-X-C motif chemokine 12.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: GBM: Glioblastoma multiforme; Alg 1%: Alginate hydrogel 1%; Alg/Chit-NPs CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); CXCL12: C-X-C motif chemokine 12.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques:

    (a) Schematic representation of the perfusion bioreactor system for GBM cells migration assay. (b) The perfusion bioreactor assembly. GBM: Glioblastoma multiforme; Alg 1%: Alginate hydrogel 1%; Alg/Chit NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); PDMS: Polydimethylsiloxane; 3D: three-dimensional.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: (a) Schematic representation of the perfusion bioreactor system for GBM cells migration assay. (b) The perfusion bioreactor assembly. GBM: Glioblastoma multiforme; Alg 1%: Alginate hydrogel 1%; Alg/Chit NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); PDMS: Polydimethylsiloxane; 3D: three-dimensional.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques: Migration

    (a) Hydrogels scans of mCherry-F98 GBM cells migration at t = 0 h, t = 24 h and t = 72 h with and without CXCL12 (0.33 μg) under a flow rate of 0.5 μL/min. (b) Hoechst staining of the hydrogels at t = 72h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 15, n = 3). Bars represent the mean ± SD and the squares are zoomed regions. Statistical analysis was performed using a Mann-Whitney t-test, * p ≤ 0.05, ** p ≤ 0.01. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: (a) Hydrogels scans of mCherry-F98 GBM cells migration at t = 0 h, t = 24 h and t = 72 h with and without CXCL12 (0.33 μg) under a flow rate of 0.5 μL/min. (b) Hoechst staining of the hydrogels at t = 72h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 15, n = 3). Bars represent the mean ± SD and the squares are zoomed regions. Statistical analysis was performed using a Mann-Whitney t-test, * p ≤ 0.05, ** p ≤ 0.01. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques: Migration, Staining, MANN-WHITNEY

    (a) Hydrogels scans of mCherry F98 GBM cells migration at t = 0 h, t = 24 h and t = 72 h with and without CXCL12 at a flow rate of 3 μL/min. (b) Hoechst staining of the hydrogels at t = 72 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was performed using a Mann-Whitney t-test, * p ≤ 0.05, ** p ≤ 0.01. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: (a) Hydrogels scans of mCherry F98 GBM cells migration at t = 0 h, t = 24 h and t = 72 h with and without CXCL12 at a flow rate of 3 μL/min. (b) Hoechst staining of the hydrogels at t = 72 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was performed using a Mann-Whitney t-test, * p ≤ 0.05, ** p ≤ 0.01. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques: Migration, Staining, MANN-WHITNEY

    (a) Hydrogels scans of U87-GFP GBM cells migration at t = 0 h, t = 24 h and t = 120 h with and without CXCL12 at a flow rate of 0.5 μL/min. (b) Hoechst staining of the hydrogels at t = 120 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was conducted using a Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 1 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) *, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) *, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) ns. 2 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) **, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 3 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) ***, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 4 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) ns, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) **. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: (a) Hydrogels scans of U87-GFP GBM cells migration at t = 0 h, t = 24 h and t = 120 h with and without CXCL12 at a flow rate of 0.5 μL/min. (b) Hoechst staining of the hydrogels at t = 120 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was conducted using a Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 1 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) *, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) *, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) ns. 2 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) **, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 3 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) ***, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 4 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) ns, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) **. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques: Migration, Staining, Comparison

    (a) Hydrogels scans of GFP U87 GBM cells migration at t = 0 h, t = 24 h and t = 120 h with and without CXCL12 at a flow rate of 3 μL/min. (b) Hoechst staining of the hydrogels at t = 120 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was conducted using a Kruskal-Wallis test followed by Dunn’s multiple comparison test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 1 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) **, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) ns. 2 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) **, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 3 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) ***, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 4 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) ns, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) **. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: (a) Hydrogels scans of GFP U87 GBM cells migration at t = 0 h, t = 24 h and t = 120 h with and without CXCL12 at a flow rate of 3 μL/min. (b) Hoechst staining of the hydrogels at t = 120 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was conducted using a Kruskal-Wallis test followed by Dunn’s multiple comparison test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 1 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) **, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) ns. 2 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) **, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 3 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) ***, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 4 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) ns, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) **. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques: Migration, Staining, Comparison

    Pathway analysis of the hits identified from the kinome and GPCR/GPCR‐associated RNAi library screens.

    Journal: Immunology and Cell Biology

    Article Title: RNAi library screening reveals Gβ1, Casein Kinase 2 and ICAP‐1 as novel regulators of LFA‐1‐mediated T cell polarity and migration

    doi: 10.1111/imcb.12838

    Figure Lengend Snippet: Pathway analysis of the hits identified from the kinome and GPCR/GPCR‐associated RNAi library screens.

    Article Snippet: Recombinant human SDF‐1α (CXCL12) and IL‐2 were from Peprotech (London, UK).

    Techniques: Activation Assay, Virus, Infection